DCA quality analysis
Four different suppliers of Sodium Dichloroacetate have been tested for toxic substances and other impurities. Chemical analysis results conclude that the purest Sodium Dichloroacetate is DCA-LAB. Products with the highest amounts of impurities are PureDCA and PharmaDCA, latter having the highest amount of toxic substances.
OBJECT OF RESEARCH
Sodium Dichloroacetate samples of four different suppliers examined:
|Brand||Company||Product||Lot/Batch number||Expiration date|
|DCA-LAB||Curaltus Ltd||25 gr. DCA Powder||Lot No: D16-C1-2/25||March, 2018|
|Sigma-Aldrich||Sigma-Aldrich Chemie GmbH||10 gr. DCA Powder||LOT# STBD9074V||-|
|Pharma DCA||A.W.P. Ltd||20 gr. DCA Powder||Batch No 01780||November, 2017|
|Pure DCA / theDCAstore||-||20 gr. DCA Powder||LOT: 72641||September, 2016|
Anionic dichloroacetate composition measured using Ionic chromatograph.
590 mg of DCA powder is dissolved in 1 lliter of distilled water (H2O conductivity - 5 mkS). Thus obtained solutions, each contained 590 mg/l sodium dichloroacetate, equivalent to 500 mg of dichloroacetate anion in 1 liter of water.
Prepared solutions (590 mg/l) examined using ionic chromatograph through an anionic column. The column and detector standardly calibrated for fluoride, chloride, nitrite, bromide, nitrate, phosphate, sulfate anions, additionally calibrated for dichloroacetate (Cl2CH-COO-), monochloroacetate (ClCH2-COO-), glyoxylate (OCH-COO-) for anions and identification peaks determined other potential impurities: glycolate (HOCH2-COO-), oxalate (-OOC-COO-) anions.
Results are presented in the table ranked from best to worst:
|Glycolate anion||Oxalate anion||Chloride anion|
|3||Pure DCA||590||446.85||0.36||0.21||Visible peak||Visible peak||Visible peak|
|4||Pharma DCA||590||484.26||1.40||-||-||Visible peak||Visible peak|
Note: Chloride anion gives a peak of 0,45 µS with a standalone water sample, it is not assessed quantitatively; it is certain that Sigma Aldrich and DCA-LAB products have an insignificant amount of chloride anion.
HEADSPACE GAS CHROMATOGRAPHY (HS-GC)
Volatile halogenated hydrocarbons (Determination of highly volatile halogenated hydrocarbon) of DCA solution measured with HS-GC chromatograph.
590 mg of DCA powder is dissolved in 1 lliter of distilled water (H2O conductivity - 5 mkS). 5 ml of the obtained solution is diluted to 500 ml (100 times), resulting in solution that contains 5,9 mg/l of sodium dichloroacetate, which is equivalent to 5 mg dichloroacetate anions in 1 liter of water.
Prepared DCA solutions (5,9 mg/l) are examined via HS-GC method, heating the solution at 90 oC temperature. This method allows to precisely examine the amount of dissolved halogenated hydrocarbons in an aqueous solution: chloroform, bromdichlormetan, chlordibrommetan, bromoform, trichloreten, tetrachloreten, at a concentration between 0.1 μg/l and 2 μg /l.
The results are presented in the table ranked from best to worst:
|Chloroform, µg/l||Bromodichloromethane, µg/l||Chlorodibromomethane, µg/l||Bromoform, µg/l||Trichloroethene, µg/l||Tetrachloroethene, µg/l|
Summarized results of the Anionic chromatography and HS-GC (Headspace Gas chromatography) presented in the table ranked from best to worst. Table displays dichloroacetate amount (%) and amounts of measured impurities: toxic monochloroacetate (ppM), toxic chloroform (ppM) and other impurities (glycolate, glyoxilate, oxalate, chloride) and H2O amount (%). Visual evidence of mechanical impurities is assessed.
anion amount, ppM
+ H2O, %
|3||Pure DCA||99||89,35||610||22||10.58||Strongly noticealbe|
|4||Pharma DCA||99||96,83||2373||107||2.92||Strongly noticealbe|
- Sodium Dichloroacetate from DCA-LAB and Sigma-Aldrich matches parameters advertised by the supplier.
- Sodium Dichloroacetate from PureDCA and PharmaDCA do not match advertised parameters.
- Purest Sodium Dichloroacetate is DCA-LAB.
- Sodium Dichloroacetate with most impurities is from PureDCA and PharmaDCA.
- Sodium Dichoroacetate from PharmaDCA has highest amounts of toxic substances.