Bo Li1,*, Xinzhe Li1,*, Zhenhong Ni1, Yan Zhang1, Yijun Zeng1, Xiaohuan Yan2, Yan Huang3, Jintao He4, Xilin Lyu1, Yaran Wu1, Yuting Wang1, Yingru Zheng2, Fengtian He1
1 Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University,
Chongqing 400038, China
2 Department of Obstetrics and Gynecology, Daping Hospital and Research Institute of Surgery, Third Military Medical
University, Chongqing 400042, China
3 Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042,
4 Battalion 17 of Students, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
* These authors have contributed equally to this work
Yingru Zheng, email: firstname.lastname@example.org
Fengtian He, email: email@example.com
Received: January 24, 2016
Accepted: July 09, 2016
Published: July 19, 2016
Both dichloroacetate (DCA) and metformin (Met) have shown promising antitumor efficacy by regulating cancer cell metabolism. However, the DCA-mediated protective autophagy and Met-induced lactate accumulation limit their tumor-killing potential respectively. So overcoming the corresponding shortages will improve their therapeutic effects. In the present study, we found that DCA and Met synergistically inhibited the growth and enhanced the apoptosis of ovarian cancer cells. Interestingly, we for the first time revealed that Met sensitized DCA via dramatically attenuating DCA-induced Mcl-1 protein and protective autophagy, while DCA sensitized Met through markedly alleviating Met-induced excessive lactate accumulation and glucose consumption. The in vivo experiments in nude mice also showed that DCA and Met synergistically suppressed the growth of xenograft ovarian tumors. These results may pave a way for developing novel strategies for the treatment of ovarian cancer based on the combined use of DCA and Met.
Keywords: dichloroacetate, metformin, Mcl-1, cancer metabolism, ovarian cancer
The mortality of ovarian cancer ranks top among several types of gynecological cancers. At present, platinum and taxol-based chemotherapies are still standard paradigm in addition to surgery, however, their side effects are severe and the chemoresistance has also emerged [1–2]. Therefore, it is urgent to explore novel strategies as alternatives of traditional chemotherapy. In recent years, the growing evidences have shown that cancer is a kind of metabolic abnormalities, which pushes it to the forefront by regulating cancer metabolism to inhibit tumor growth . Targeting key metabolic pathways significantly kill numerous cancer cells including ovarian cancer cells [4–5]. Among various metabolic drugs, dichloroacetate (DCA) and metformin (Met) have shown charming prospects because of their positive functions in cancer therapy.
As a mitochondria-targeting agent, DCA can inhibit the activity of pyruvate dehydrogenase kinase (PDK) and subsequently increase the activity of pyruvate dehydrogenase (PDH), which promotes the flux of carbohydrates into mitochondria and thereby enhances aerobic oxidation of glucose. This effect reverses mitochondrial dysfunction and reactivates mitochondria-dependent apoptosis in several tumor cells [6–9]. Simultaneously, DCA inhibits glycolysis and reduces lactate accumulation, which destroys the acidified tumor microenvironment (The acidified microenvironment is generally in favor of tumor survival) . Although DCA has shown promising prospect in fighting against cancers, it has been reported that DCA induces protective autophagy in colon cancer cells which in turn hinders its apoptotic capacity . So far, it is still unclear whether there is any other apoptosis-associated resistant determinant when DCA refreshes mitochondrial apoptosis.
Met is a traditional drug of first-line therapy for type 2 diabetes. Recent years, increasing evidences indicate that Met can also reduce the risk of cancer in several epidemiological studies . Met suppresses tumor growth through inducing cycle arrest, promoting apoptosis and suppressing autophagy [13–15]. Furthermore, Met can sensitize some chemotherapeutic drugs such as paclitaxel, erlotinib, etc. [16–17]. More arrestingly, the anti-tumor effect of Met is increasingly linked to the glucose metabolism of cancer . Despite several advantages in clinical trials, Met is hampered for further application because it might lead to lactate accumulation . It is of great interest whether this disadvantage could be overcome by combining other metabolic drugs to make Met more extensively used in chemotherapy.
Given their potential mutual compensatory effects, we sought to uncover whether DCA and Met can synergize each other to enhance cytotoxicity in ovarian cancer cells. In the present study, we demonstrated that DCA and Met could collaboratively induce apoptosis in ovarian cancer cells. Met sensitized DCA via dramatically attenuating DCA-induce Mcl-1 and protective autophagy, while DCA sensitized Met through markedly alleviated Met-induced excessive lactate accumulation and glucose consumption. The in vivo experiments in nude mice also showed that DCA and Met synergistically suppressed the growth of xenograft ovarian tumors. These results suggest that this therapeutic strategy may be a promising choice for future metabolism-based targeted cancer therapy.
DCA and Met synergistically induce apoptosis in ovarian cancer cells
To investigate whether there is a synergistic effect between DCA and Met in suppressing the growth of ovarian cancer cells, SKOV3 and OVCAR3 cells were cotreated with DCA and Met or each alone. As shown in Figure 1A-1C, cotreatment with DCA and Met more efficiently repressed the growth of the ovarian cancer cells compared to each alone, and the combination of 40mM DCA and 10mM Met could inhibit the cell viability to as low as 50% compared to the control. So we chose 40mM DCA and 10mM Met in the subsequent experiments. Similarly, the synergistic inhibition effect was also observed in cervical cancer cells (HeLa and SiHa), non-small cell lung cancer cells (A549 and GLC-82) and human hepatocellular carcinoma cells (HepG2) (Figure S1A-1D), suggesting that the synergism between DCA and Met may be universal to some extent. Moreover, DCA and Met synergistically induced apoptosis in ovarian cancer cells revealed by Flow Cytometry analysis of annexin V-FITC (fluorescein isothiocyanate) and PI (prodium iodide) double staining (Figure 1D), Hoechst staining of apoptotic bodies (Figure 1E), Western blot analysis of cleaved PARP (poly ADP-ribose polymerase, a marker of apoptosis) (Figure 1F) and caspase3 activity assay (Figure 1G).
Met sensitizes DCA via attenuating DCA-induced Mcl-1
To explore the mechanism by which Met sensitizes DCA to induce apoptosis, we examined the expression of the crucial antiapoptotic Bcl-2 family members including Mcl-1, Bcl-2 and Bcl-xL . As shown in Figure 2A, DCA alone significantly increased the level of Mcl-1 protein (but not Bcl-2 and Bcl-xL proteins) in ovarian cancer cells, which was markedly attenuated by Met. Silence of Mcl-1 by siRNA enhanced the DCA-mediated inhibition of the cell viability (Figure 2B), and augmented DCA-induced apoptosis (Figure 2C-2E). Moreover, ectopic expression of Mcl-1 dramatically alleviated the sensitizing effect of Met to DCA on cell viability and apoptosis (Figure 2F-2I). These results indicated that Mcl-1 is a novel resistant factor of DCA, and Met sensitizes DCA via downregulating Mcl-1.
Met attenuates DCA-induced Mcl-1 through inhibiting Mcl-1 translation
To elucidate in which level DCA induces Mcl-1, the mRNA of Mcl-1 was firstly examined. As shown in Figure 3A, DCA had no obvious effect on Mcl-1 mRNA expression. Subsequently, Mcl-1 protein was analyzed in the presence or absence of translational inhibitor cycloheximide (CHX). As shown in Figure 3B and 3C, CHX time-dependently decreased the basal (but not DCA-induced) Mcl-1 protein, indicating that DCA increases the stability of Mcl-1 protein. It has been reported that the phosphorylated ERK (p-ERK) and p-JNK can stabilize Mcl-1 through phosphorylating Mcl-1 on Thr163 [21–22], so we investigated whether p-ERK/p-JNK is involved in the regulation of DCA-induced Mcl-1. As shown in Figure 3D, treatment with DCA significantly elevated p-ERK (but not p-JNK) and p-Mcl-1Thr163 in ovarian cancer cells. Moreover, the MEK1/2 inhibitor U0126 could dramatically attenuated DCA-induced Mcl-1 and p-Mcl-1Thr163, and markedly strengthened the cleaved PARP (Figure 3E). These results indicated that p-ERK (but not p-JNK) is a mediator of DCA-induced Mcl-1. Previous studies have demonstrated that DCA elevates Reactive Oxygen Species (ROS) , and ROS is a key inducer of p-ERK , so we examined the level of ROS with DCFH-DA. As shown in Figure S2A, DCA increased the generation of ROS, suggesting that induction of ROS may be a mechanism by which DCA enhances p-ERK activation.
Additionally, Ser159 is also closely related with Mcl-1 stability and this site is mainly phosphorylated by GSK-3β , so we tested whether GSK-3β is also involved in the regulation of DCA-induced Mcl-1 stabilization. As shown in Figure S2B, DCA increased the phosphorylation of GSK-3β, but had no effect on total GSK-3β. Moreover, DCA enhanced the phosphorylation of Akt (an upstream signal molecule of GSK-3β), and Akt inhibitor MK-2206 2HCl dramatically attenuated DCA-induced GSK-3β phosphorylation, Mcl-1 upregulation and apoptosis resistance (Figure S2C). These results indicated that p-Akt-mediated phosphorylation of GSK-3β promotes the DCA-induced Mcl-1 stabilization.
Subsequently, we examined whether Met can attenuate DCA-induced Mcl-1 by inhibiting p-ERK/p-Akt. As shown in Figure 3F and Figure S2D, Met could not suppress the DCA-induced p-ERK and p-Akt, together with the results in Figure 3A, indicating that Met decreases DCA-induced Mcl-1 neither at transcriptional nor at post-translational level. Then we analyzed whether Met attenuates DCA-induced Mcl-1 at translational level with the proteasome inhibitor MG132. As shown in Figure 3G, when cells were treated with control, DCA, Met or combination, the protein levels of Mcl-1 were equally elevated in the presence of MG132 compared to DMSO, indicating that Met attenuates DCA-induced Mcl-1 via inhibiting Mcl-1 translation. It has been reported activation of mTOR promotes Mcl-1 translation , so we analyzed p-mTOR after cotreatment with Met and DCA. As expected, Met markedly decreased the level of p-mTOR (Figure 3H), and the mTOR inhibitor PP242 had the similar effect to Met on promoting apoptosis (Figure 3I). According to the data in Figure 3 and Figure S2, we can conclude that DCA upregulates Mcl-1 via enhancing the phosphorylation of ERK and GSK-3β, and Met suppresses Mcl-1 translation through inhibiting p-mTOR.
Met diminishes DCA-induced protective autophagy
Previous studies have shown that autophagy plays an important role in the therapeutic resistance of DCA in colon cancer cells , so we examined the role of autophagy in ovarian cancer cells upon treatment with DCA or/and Met. As shown in Figure 4A, DCA dose-dependently promoted the level of MAP1LC3-II (LC3-II), the marker of autophagy. Inhibition of autophagy by chloroquine (CQ) or silence of ATG7 dramatically enhanced the DCA-induced apoptosis and cytotoxicity (Figure 4B-4D, Figure S3A-3D), indicating that DCA induces protective autophagy in ovarian cancer cells. To preliminary investigate the mechanism of DCA-induced autophagy, we scanned the mRNA changes of 7 autophagy-related genes in DCA-treated cells. As shown in Figure S3E, DCA dramatically upregulated the mRNA level of ATG7 in ovarian cancer cells, suggesting that ATG7 may be involved in the DCA-induced protective autophagy. Subsequently, we found that Met remarkably decreased the DCA-induced LC3-II (Figure 4E-4G), indicating that Met could attenuate the DCA-induced protective autophagy. In summary, it could be drawn that weakening DCA-induced protective autophagy is also important in the sensitizing effect of Met to DCA.
DCA alleviates Met-induced glucose consumption and lactate production
To clarify whether DCA can overcome the shortages of Met, the changes of lactate and glucose were analyzed. As shown in Figure 5A-5C, Met increased lactate production and glucose consumption, which was remarkably alleviated by DCA. Moreover, the cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured. As shown in Figure 5D-5F, Met decreased the ratio of OCR/ECAR, which was dramatically attenuated by DCA, revealing that DCA can suppress the Met-induced glycolysis via recovering mitochondrial respiration. As DCA is an inhibitor of PDKs which phosphorylates and inhibits the activity of PDH , so we examined the level of p-PDH. As shown in Figure 5G, Met elevated the level of p-PDHE1α (a subunit of PDH), which was markedly reversed by DCA. Silence of PDH with siRNA significantly attenuated the Met-induced lactate production (Figure 5H, 5I). Ectopic expression of PDK1 and PDK2 enhanced the phosphrylation of PDHE1α and attenuated apoptosis induced by the cotreatment with DCA and Met (Figure 5J, 5K), taken together with the data in Figures 5G-5I, indicating that DCA can sensitize Met through inhibiting PDK/PDH pathway in killing ovarian cancer cells.
DCA and Met collaboratively repress the growth of ovarian cancer cells in vivo
As shown in Figure 6A and 6B, cotreatment with DCA and Met more efficiently suppressed the growth of ovarian cancer xenografts in nude mice compared to the treatment with DCA or Met alone. Western blot analysis showed that DCA and Met synergistically increased cleaved PARP, and downregulated Mcl-1 and p-PDHE1α in the xenografts (Figure 6C-6D). These results suggest that DCA and Met can collaboratively inhibit the growth of ovarian cancer cells in vivo through attenuating the shortages of each other.
It has been confirmed that most solid tumors are characterized by “Warburg effect” whereby they use glycolysis for energy production even though oxygen is sufficient. Targeting this abnormal phenomenon has paved a way for developing novel cancer therapeutic strategies in addition to traditional cytotoxic drugs. In the present study, we demonstrated that cotreatment with DCA and Met (two metabolic-associated agents) can more efficiently repress the growth of ovarian cancer cells compared to each alone in vitro and in vivo. Met attenuates DCA-induced Mcl-1 and protective autophagy, while DCA alleviates Met-induced excessive lactate accumulation and glucose consumption. The reciprocal benefits of the two agents contribute an intense apoptosis to kill ovarian cancer cells more effectively. The working model of DCA and Met in combination was shown in Figure 7.
Although studies have been undergoing for years, the key factors which may impede the pro-apoptotic effect of DCA are not yet clear. Our results showed that Mcl-1 is a crucial resistant factor against DCA-induced apoptosis in ovarian cancer cells, and cotreatment with Met and DCA decreased Mcl-1 and enhanced apoptosis. However, the cotreatment led to an increase of Bcl-xL (Figure 2A), which may be as a compensatory mechanism to keep the cell survival. The similar phenomenon has also been reported in previous study  (The study shows that Bcl-2/xL inhibitor ABT-263 induces cancer cell apoptosis while upregulates Mcl-1). Moreover, the cotreatment-induced Bcl-xL increase was only present in SKOV3 (but not in OVCAR3) cell line, suggesting that this effect may be cell specific. Of course, it remains to be determined about the detailed mechanism by which the cotreatment with Met and DCA upregulates Bcl-xL. By further study, we revealed that DCA induced Mcl-1 accumulation via activating ERK and Akt, which protected Mcl-1 from proteasome-mediated degradation. These findings suggest that inhibition of ERK and Akt may be a good strategy to sensitize DCA in ovarian cancer treatment. However, a conflict result has been reported recently that DCA decreases the level of Mcl-1 in AML cells  and colorectal cancer cells . The discrepancies suggest that the relationship between DCA and Mcl-1 may be largely different in different contexts.
Autophagy is a catabolic process to recycle essential metabolites such as amino acids and lipids for replenishing their bioenergetic reserve in the presence of nutrients deprivation or other dramatic stresses [30–31]. In this study, we revealed that DCA induced a protective autophagy in ovarian cancer cells, and ATG7 may play a role in this process (Figure S3E), but the detailed mechanism needs to be further studied. Moreover, we found that Met sensitized DCA via suppressing the DCA-induced protective autophagy. Consistent with our findings, Met can repress GRP78-dependent autophagy to enhance the anti-myeloma effect of bortezomib , and inhibit 2DG-induced autophagy to sensitize 2DG in prostate cancer cells . However, the detailed mechanism(s) by which Met suppresses the protective autophagy remains to be further investigated.
Met has been well recognized as a single agent or sensitizer in cancer therapy, but a notable disadvantage is that Met promotes glucose consumption and accelerates lactate accumulation which facilitates cancer-addicted aerobic glycolysis. In the present study, we demonstrated that DCA could dramatically attenuate this side effect. DCA can strongly inhibits the activity of PDKs and its downstream p-PDHE1α, leading to a metabolic remodeling reusing oxidative phosphorylation and causing lower lactate accumulation and glucose consumption. Among the four types of PDK isoenzymes, DCA mainly functions through inhibiting PDK2 and PDK1 . As expected, concurrent over-expression of PDK2 and PDK1 enhanced the phosphorylation of PDHE1α, abolished the sensitizing function of DCA and partially abrogated the lethal effect of DCA plus Met in ovarian cancer cells. Taken together, our results indicate that DCA sensitizes the anti-tumor function of Met through inhibiting the activity of PDKs. However, it should be noted that DCA can sensitize Met by enhancing Met-induced oxidative stress in breast cancer cells . This means the synergizing mechanism of DCA to Met is so complicated, which needs deep studies.
In summary, we showed that DCA and Met can synergistically suppress the growth of ovarian cancer cells, which may pave a way for developing novel strategies for the treatment of ovarian cancer based on the combined use of DCA and Met.
MATERIALS AND METHODS
Cell lines and reagents
The cell lines including SKOV3, OVCAR3, HeLa, SiHa, GLC-82, A549 and HepG2 were purchased from American Type Culture Collection (ATCC) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM), supplemented with 10% fetal bovine serum (FBS), streptomycin (100 mg/mL) and penicillin (100 U/mL) at 37°C in a 5% CO2 humid incubator. DCA and Cycloheximide (CHX) were purchased from Sigma-Aldrich (Louis, MO, USA). Met, U0126, MG132 and Hoechst 33258 were purchased from Beyotime Company (Shanghai, China). MK2206 was purchased from Selleck Company (Shanghai, China). Caspase-3 Activity Assay Kit and Reactive Oxygen Species Assay Kit were purchased from Beyotime Company (Shanghai, China). Annexin V-FITC and PI were purchased from BD Bioscience (BD, NJ, USA). siRNAs to Mcl-1, ATG7, PDH and control siRNA were from GenePharma (Shanghai, China). pCMV and pcDNA3.1, pCMV-Mcl-1, pcDNA3.1-PDK1 and pcDNA3.1-PDK2 expression plasmids were bought from Obio Technology (Shanghai, China).
Whole cell lysates were prepared and Western blot was performed as previously described . The antibodies for β-actin and PDHE1α were from Abcam Company (San Francisco, CA, USA), antibodies for Bcl-2 and Mcl-1were from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and the antibodies for PARP, BCL-xL, GSK-3β, p-ERK (Thr202/Tyr204), p-JNK (Thr183/Tyr185), p-Mcl-1 (Thr163), p-Akt (Ser473), p-4EBP1, p-mTOR and p-GSK-3β (Ser9) were from Cell Signaling Technology (Boston, MA, USA). The antibody against p-PDHE1α (Ser293) was from EMD Millipore (Billerica, MA, USA).
SKOV3 and OVCAR3 cells were grown to 60% to 70% confluence in 6-well plates. The siRNA or expression plasmid was mixed with 10 μL lipofectamine 2000 in Opti-MEM (Invitrogen, Carlsbad, CA, USA) for each well according to the manufacturer’s protocol. After incubated with the mixtures for 6 h, the cells were cultured in DMEM with 10% FBS for another 6 h. Then the cells were given the corresponding treatment.
Cell viability assay
Cell viability assay was performed using CCK-8 kit (Dojindo, Shanghai, China) as previously described . Briefly, cells were seeded onto 96-well plates (2×103 cells/well) in triplicate and incubated for 12 h. Then the cells were given different treatment or vehicle control for 48 h, followed by the addition of 10 μl CCK-8 solution to each well. After incubation at 37°C for 1.5 h, the value of OD450nm was determined with a microplate reader.
Ovarian cancer cells were incubated with annexin V-FITC and PI according to the manufacturer’s instructions (BD, 561012). Then the apoptosis were analyzed by a flow cytometer.
After treated for 24 h, the cells were stained with Hoechst 33258 (Beyotime, Shanghai, China) at 10μg/mL for 10 min at dark. Subsequently, the cells were washed 3 times with PBS and photographed under afluorescence microscope.
Caspase 3 activity assay
Caspase 3 activity was examined with Caspase3 Activity Assay kit (Beyotime, C1115) as previously described . Briefly, the control and treated cells were harvested, washed with ice-cold PBS, and resuspended in 50 μl of chilled cell lysis buffer for 15 min on ice. Then the lysates were centrifuged (20,000 g, 10 min, 4°C), and the supernatants were collected for caspase3 activity assay immediately.
Measurement of total intracellular ROS
The total intracellular ROS was tested with ROS Assay Kit (Beyotime, Shanghai, China) as previously described .
RNA isolation, quantitative real-time PCR (qPCR)
Total RNA was extracted from the cells with TRIzol reagent (ComWin Biotechnology, Beijing, China) and the first-strand cDNA was synthesized using M-MLV transcriptase (Invitrogen, Carlsbad, CA, USA). The qPCR was done with QuantiFast SYBR Green PCR Kit (Promega, Shanghai, China). The relative mRNA levels of the target genes were calculated with 2−ΔΔCt method.
Analysis of green fluorescent protein (GFP)-MAP1LC3
After transfected with the GFP-MAP1LC3 (GFP-LC3) expression vector for 12h, the cells were given the indicated treatments for another 24 h and then fixed with 4% formaldehyde for 10 min. Subsequently, the cells were washed 3 times with PBS and observed under a fluorescent microscope.
Detection of L-lactate and glucose
The cells were treated in 6-wells, and then the medium was collected and the concentrations of L-lactate and glucose were determined separately using L-Lactate Assay Kit (Eton Bioscience, San Diego, CA, USA) and Glucose Colorimetric/Fluorometic Assay Kit (BioVision, Milpitas, CA, USA).
Cellular bioenergetics analysis
The cells were plated in XF96 plates and allowed to grow overnight. Then the media were replaced with XF96 media 1 h before the assay. Rotenone/antimycin A, FCCP, and oligomycin were diluted into XF96 media and loaded into the accompanying cartridge to achieve final concentrations of 0.5μM, 0.5μM, and 1.0μM, respectively. Injections of the drugs into the medium occurred at the time points specified. The OCR (pmol/min) and ECAR (mpH/min) were monitored with the XF Cell Mito Stress Test Kit (Seahorse Bioscience, North Billerica, MA, USA) using Seahorse Bioscience XFe and XF Extracellular Flux Analyzers.
Six-week-old female nude mice were bought from Beijing Huafukang Bioscience (Beijing, China), and housed and cared for under the regulations of the guidelines of the Animal Care and Ethics Committee of Third Military Medical University (Chongqing, China). 5×106 SKOV3 cells in 150 μL PBS were implanted into the right axillae of each nude mouse. When palpable tumors were formed, the mice were randomized into 4 groups (n = 6 per group). Then the mice were intraperitoneally injected everyday with DCA (50 mg/kg/d) plus Met (100 mg/kg/d) or each alone for 8 days, taking PBS as control. The xenograft tumor size was monitored every day with sliding caliper, and the volume was estimated using the following formula: volume = width2×length×1/2. After excised from the mice, the xenograft tumors were photographed, and the corresponding proteins were examined by Western blot.
The data were expressed as mean ± SD. One-way ANOVA and t-test were used to analyze the variance. P< 0.05 was considered as statistical significant.
This work was supported by the National Natural Science Foundation of China (81472436 and 81272865) and the Natural Science Foundation of Chongqing (cstc2012jjB10025).
CONFLICTS OF INTEREST
No potential conflicts of interest were disclosed.
REFERENCES1 Jayson GC, Kohn EC, Kitchener HC, Ledermann JA. Ovarian cancer. Lancet. 2014; 384:1376-1388.
2 Patch AM, Christie EL, Etemadmoghadam D, Garsed DW, George J, Fereday S, Nones K, Cowin P, Alsop K, Bailey PJ, Kassahn KS, Newell F, Quinn MC, et al. Whole-genome characterization of chemoresistant ovarian cancer. Nature. 2015; 521:489-494.
3 Ward PS, Thompson CB. Metabolic reprogramming: a cancer hallmark even warburg did not anticipate. Cancer Cell. 2012; 21:297-308.
4 Mandai M, Amano Y, Yamaguchi K, Matsumura N, Baba T, Konishi I. Ovarian clear cell carcinoma meets metabolism; HNF-1beta confers survival benefits through the Warburg effect and ROS reduction. Oncotarget. 2015; 6:30704-30714. doi: 10.18632/oncotarget.5228.
5 Fan JY, Yang Y, Xie JY, Lu YL, Shi K, Huang YQ. MicroRNA-144 mediates metabolic shift in ovarian cancer cells by directly targeting Glut1. Tumour Biol. 2015.
6 Michelakis ED, Sutendra G, Dromparis P, Webster L, Haromy A, Niven E, Maguire C, Gammer TL, Mackey JR, Fulton D, Abdulkarim B, McMurtry MS, Petruk KC. Metabolic modulation of glioblastoma with dichloroacetate. Sci Transl Med. 2010; 2:31ra34.
7 Michelakis ED, Webster L, Mackey JR. Dichloroacetate (DCA) as a potential metabolic-targeting therapy for cancer. Br J Cancer. 2008; 99:989-994.
8 Papandreou I, Goliasova T, Denko NC. Anticancer drugs that target metabolism: Is dichloroacetate the new paradigm? Int J Cancer. 2011; 128:1001-1008.
9 Bonnet S, Archer SL, Allalunis-Turner J, Haromy A, Beaulieu C, Thompson R, Lee CT, Lopaschuk GD, Puttagunta L, Harry G, Hashimoto K, Porter CJ, Andrade MA, et al. A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell. 2007; 11:37-51.
10 Ohashi T, Akazawa T, Aoki M, Kuze B, Mizuta K, Ito Y, Inoue N. Dichloroacetate improves immune dysfunction caused by tumor-secreted lactic acid and increases antitumor immunoreactivity. Int J Cancer. 2013; 133:1107-1118.
11 Gong F, Peng X, Sang Y, Qiu M, Luo C, He Z, Zhao X, Tong A. Dichloroacetate induces protective autophagy in LoVo cells: involvement of cathepsin D/thioredoxin-like protein 1 and Akt-mTOR-mediated signaling. Cell Death Dis. 2013; 4:e913.
12 Morales DR, Morris AD. Metformin in cancer treatment and prevention. Annu Rev Med. 2015; 66:17-29.
13 Ben Sahra I, Laurent K, Loubat A, Giorgetti-Peraldi S, Colosetti P, Auberger P, Tanti JF, Le Marchand-Brustel Y, Bost F. The antidiabetic drug metformin exerts an antitumoral effect in vitro and in vivo through a decrease of cyclin D1 level. Oncogene. 2008; 27:3576-3586.
14 Liu B, Fan Z, Edgerton SM, Deng XS, Alimova IN, Lind SE, Thor AD. Metformin induces unique biological and molecular responses in triple negative breast cancer cells. Cell Cycle. 2009; 8:2031-2040.
15 Jagannathan S, Abdel-Malek MA, Malek E, Vad N, Latif T, Anderson KC, Driscoll JJ. Pharmacologic screens reveal metformin that suppresses GRP78-dependent autophagy to enhance the anti-myeloma effect of bortezomib. Leukemia. 2015; 29:2184-2191.
16 Rocha GZ, Dias MM, Ropelle ER, Osorio-Costa F, Rossato FA, Vercesi AE, Saad MJ, Carvalheira JB. Metformin amplifies chemotherapy-induced AMPK activation and antitumoral growth. Clin Cancer Res. 2011; 17:3993-4005.
17 Lau YK, Du X, Rayannavar V, Hopkins B, Shaw J, Bessler E, Thomas T, Pires MM, Keniry M, Parsons RE, Cremers S, Szabolcs M, Maurer MA. Metformin and erlotinib synergize to inhibit basal breast cancer. Oncotarget. 2014; 5:10503-10517. doi: 10.18632/oncotarget.2391.
18 Scotland S, Saland E, Skuli N, de Toni F, Boutzen H, Micklow E, Senegas I, Peyraud R, Peyriga L, Theodoro F, Dumon E, Martineau Y, Danet-Desnoyers G, et al. Mitochondrial energetic and AKT status mediate metabolic effects and apoptosis of metformin in human leukemic cells. Leukemia. 2013; 27:2129-2138.
19 Chaube B, Malvi P, Singh SV, Mohammad N, Meena AS, Bhat MK. Targeting metabolic flexibility by simultaneously inhibiting respiratory complex I and lactate generation retards melanoma progression. Oncotarget. 2015; 6:37281-37299. doi: 10.18632/oncotarget.6134.
20 Lessene G, Czabotar PE, Colman PM. BCL-2 family antagonists for cancer therapy. Nat Rev Drug Discov. 2008; 7:989-1000.
21 Domina AM, Vrana JA, Gregory MA, Hann SR, Craig RW. MCL1 is phosphorylated in the PEST region and stabilized upon ERK activation in viable cells, and at additional sites with cytotoxic okadaic acid or taxol. Oncogene. 2004; 23:5301-5315.
22 Kodama Y, Taura K, Miura K, Schnabl B, Osawa Y, Brenner DA. Antiapoptotic effect of c-Jun N-terminal Kinase-1 through Mcl-1 stabilization in TNF-induced hepatocyte apoptosis. Gastroenterology. 2009; 136:1423-1434.
23 Roh JL, Park JY, Kim EH, Jang HJ, Kwon M. Activation of mitochondrial oxidation by PDK2 inhibition reverses cisplatin resistance in head and neck cancer. Cancer Lett. 2016; 371:20-29.
24 Li X, Wang K, Ren Y, Zhang L, Tang XJ, Zhang HM, Zhao CQ, Liu PJ, Zhang JM, He JJ. MAPK signaling mediates sinomenine hydrochloride-induced human breast cancer cell death via both reactive oxygen species-dependent and -independent pathways: an in vitro and in vivo study. Cell Death Dis. 2014; 5:e1356.
25 Maurer U, Charvet C, Wagman AS, Dejardin E, Green DR. Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1. Mol Cell. 2006; 21:749-760.
26 Mills JR, Hippo Y, Robert F, Chen SM, Malina A, Lin CJ, Trojahn U, Wendel HG, Charest A, Bronson RT, Kogan SC, Nadon R, Housman DE, et al. mTORC1 promotes survival through translational control of Mcl-1. Proc Natl Acad Sci U S A. 2008; 105:10853-10858.
27 Wang B, Ni Z, Dai X, Qin L, Li X, Xu L, Lian J, He F. The Bcl-2/xL inhibitor ABT-263 increases the stability of Mcl-1 mRNA and protein in hepatocellular carcinoma cells. Mol Cancer. 2014; 13:98.
28 Emadi A, Sadowska M, Carter-Cooper B, Bhatnagar V, van der Merwe I, Levis MJ, Sausville EA, Lapidus RG. Perturbation of cellular oxidative state induced by dichloroacetate and arsenic trioxide for treatment of acute myeloid leukemia. Leuk Res. 2015; 39:719-729.
29 Delaney LM, Ho N, Morrison J, Farias NR, Mosser DD, Coomber BL. Dichloroacetate affects proliferation but not survival of human colorectal cancer cells. Apoptosis. 2015; 20:63-74.
30 Mah LY, Ryan KM. Autophagy and cancer. Cold Spring Harb Perspect Biol. 2012; 4:a008821.
31 Yang ZJ, Chee CE, Huang S, Sinicrope FA. The role of autophagy in cancer: therapeutic implications. Mol Cancer Ther. 2011; 10:1533-1541.
32 Ben Sahra I, Laurent K, Giuliano S, Larbret F, Ponzio G, Gounon P, Le Marchand-Brustel Y, Giorgetti-Peraldi S, Cormont M, Bertolotto C, Deckert M, Auberger P, Tanti JF, et al. Targeting cancer cell metabolism: the combination of metformin and 2-deoxyglucose induces p53-dependent apoptosis in prostate cancer cells. Cancer Res. 2010; 70:2465-2475.
33 Bowker-Kinley MM, Davis WI, Wu P, Harris RA, Popov KM. Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complex. Biochem J. 1998; 329:191-196.
34 Haugrud AB, Zhuang Y, Coppock JD, Miskimins WK. Dichloroacetate enhances apoptotic cell death via oxidative damage and attenuates lactate production in metformin-treated breast cancer cells. Breast Cancer Res Treat. 2014; 147:539-550.
35 Lian J, Ni Z, Dai X, Su C, Smith AR, Xu L, He F. Sorafenib sensitizes (-)-gossypol-induced growth suppression in androgen-independent prostate cancer cells via Mcl-1 inhibition and Bak activation. Mol Cancer Ther. 2012; 11:416-426.
36 Ou X, Lu Y, Liao L, Li D, Liu L, Liu H, Xu H. Nitidine chloride induces apoptosis in human hepatocellular carcinoma cells through a pathway involving p53, p21, Bax and Bcl-2. Oncol Rep. 2015; 33:1264-1274.
37 Ni Z, Gong Y, Dai X, Ding W, Wang B, Gong H, Qin L, Cheng P, Li S, Lian J, He F. AU4S: a novel synthetic peptide to measure the activity of ATG4 in living cells. Autophagy. 2015; 11:403-415.